Biological Activity
Irreversible inhibitor of aspartic proteases. Inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with E 64d (Cat. No. 4545).
Technical Data
| M. Wt | 685.89 |
| Formula | C34H63N5O9 |
| Sequence |
VVA (Modifications: Val-1 = N-terminal H-iso-Valeryl, Val-2 = Val-4-amino-3-hydroxy-6-methylheptanoyl, Ala-3 = Ala-4-amino-3-hydroxy-6-methylheptanoic-acid) |
| Storage | Desiccate at -20°C |
| CAS Number | 26305-03-3 |
| PubChem ID | 5478883 |
| InChI Key | FAXGPCHRFPCXOO-LXTPJMTPSA-N |
| Smiles | O=C(N[C@@H](CC(C)C)[C@@H](O)CC(N[C@@H](C)C(N[C@H]([C@@H](O)CC(O)=O)CC(C)C)=O)=O)[C@H]([C@@H](C)C)NC([C@@H](NC(CC(C)C)=O)C(C)C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 1 mg/ml in ethanol with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 685.89. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.46 mL | 7.29 mL | 14.58 mL |
| 5 mM | 0.29 mL | 1.46 mL | 2.92 mL |
| 10 mM | 0.15 mL | 0.73 mL | 1.46 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.29 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Marciniszyn et al (1976) Mode of inhibition of acid proteases by pepstatin. J.Biol.Chem. 251 7088 PMID: 993206
Merck Index 12 7290 PMID:
Sato et al (2007) Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation. Cancer Res. 67 9677 PMID: 17942897
Keywords: Pepstatin A, supplier, Aspartic, protease, inhibitors, inhibits, Proteases, Proteinases, PepstatinA, Other, Proteases, Autophagy, Other, Proteases, Tocris Bioscience
