Biological Activity
Extensively used phorbol ester activator of protein kinase C; tumor promoter. Binds to PKC with a Ki value of 2.6 nM as measured by displacement of [3H]phorbol 12,13-dibutyrate binding in rat cortex synaptosomal membranes.
Technical Data
| M. Wt | 616.83 |
| Formula | C36H56O8 |
| Storage | Desiccate at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 16561-29-8 |
| PubChem ID | 27924 |
| InChI Key | PHEDXBVPIONUQT-RGYGYFBISA-N |
| Smiles | OCC1=C[C@]([C@@](C(C)4C)([H])[C@@]4(OC(C)=O)[C@H](OC(CCCCCCCCCCCCC)=O)[C@H]3C)([H])[C@@]3(O)[C@@](C=C(C)C2=O)([H])[C@]2(O)C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 61.68 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 616.83. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.62 mL | 8.11 mL | 16.21 mL |
| 5 mM | 0.32 mL | 1.62 mL | 3.24 mL |
| 10 mM | 0.16 mL | 0.81 mL | 1.62 mL |
| 50 mM | 0.03 mL | 0.16 mL | 0.32 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Castagna et al (1982) Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J.Biol.Chem. 257 7847 PMID: 7085651
Hockberger et al (1989) A diacylglycerol analogue reduces neuronal calcium currents independently of protein kinase C activation. Nature 338 340 PMID: 2922062
Murphy et al (1999) Structural determinants of phorbol ester binding in synaptosomes: pharmacokinetics and pharmacodynamics. Eur.J.Pharmacol. 381 77 PMID: 10528137
Merck Index 12 7487 PMID:
Keywords: Phorbol 12-myristate 13-acetate, supplier, Protein, kinases, C, activators, PKC, Phorbol12-myristate13-acetate, PMA, Protein, Kinase, C, Protein, Kinase, C, Tocris Bioscience
