Biological Activity
Dual inhibitor of extracellular signal-regulated kinase 1 (ERK1, MAPK3) and RasGAP. Maintains embryonic stem cell (ESC) self-renewal. Enables propagation of undifferentiated murine ESCs in the absence of leukemia inhibitory factor (LIF).
Technical Data
| M. Wt | 550.54 |
| Formula | C27H25F3N8O2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 839707-37-8 |
| PubChem ID | 12003241 |
| InChI Key | NBZFRTJWEIHFPF-UHFFFAOYSA-N |
| Smiles | CN2C1=NC(NC4=CC(C)=NN4C)=NC=C1CN(C3=CC(NC(C5=CC(C(F)(F)F)=CC=C5)=O)=CC=C3C)C2=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 13.76 | 25 |
Preparing Stock Solutions
The following data is based on the product molecular weight 550.54. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.82 mL | 9.08 mL | 18.16 mL |
| 5 mM | 0.36 mL | 1.82 mL | 3.63 mL |
| 10 mM | 0.18 mL | 0.91 mL | 1.82 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.36 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Wu and Ding et al (2006) Applying chemical tools to the discovery of novel regenerative medicine. Drug Discov.Today Technol. 3 255 PMID:
Chen et al (2006) Self-renewal of embryonic stem cells by a small molecule. Proc.Natl.Acad.Sci.USA 103 17266 PMID: 17088537
Mertins et al (2013) A small molecule (pluripotin) as a tool for studying cancer stem cell biology: proof of concept. PLoS One 8 e57099 PMID: 23437320
Keywords: Pluripotin, supplier, dual, inhibitors, inhibits, extracellular, signal-regulated, kinase, 1, ERK1, MAPK3, mitogen-activated, protein, kinase, 3, RasGAP, mouse, murine, stem, cells, self-renewal, SC1, SC1, ERK, Stem, Cell, Proliferation, ERK, Tocris Bioscience
