Biological Activity
Highly potent neurosteroid; enhances intracellular Ca2+ upon NMDA receptor-mediated synaptic activity (EC50 = ~2 pM) and potentiates NMDA responses. Activates CREB phosphorylation, augments LTP and cognitive behavior in rat memory models. Also activates TRPM3 channels.
Technical Data
| M. Wt | 418.52 |
| Formula | C21H31NaO5S |
| Storage | Store at +4°C |
| CAS Number | 1852-38-6 |
| PubChem ID | 23676306 |
| InChI Key | QQVJEIZJHDPTSH-UTNKIXDHSA-M |
| Smiles | O=[C@](C)[C@H]4CC[C@@]3([H])[C@]2([H])CC=C1C[C@@H](OS(=O)([O-])=O)CC[C@@](C)1[C@]([H])2CC[C@@]34C.[Na+] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 41.85 | 100 | |
| water | 4.19 | 10mM with sonication |
Preparing Stock Solutions
The following data is based on the product molecular weight 418.52. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.39 mL | 11.95 mL | 23.89 mL |
| 5 mM | 0.48 mL | 2.39 mL | 4.78 mL |
| 10 mM | 0.24 mL | 1.19 mL | 2.39 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
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References
References are publications that support the products' biological activity.
Li et al (2007) Neuroactive steroids and human recombinant rho1 GABAC receptors. J.Pharmacol.Exp.Ther. 323 236 PMID: 17636008
Majeed et al (2010) Cis-isomerism and other chemical requirements of steroidal agonists and partial agonists acting at TRPM3 channels. Br.J.Pharmacol. 161 430 PMID: 20735426
Smith et al (2014) A role for picomolar concentrations of pregnenolone sulfate in synaptic activity-dependent Ca2+ signaling and CREB activation. Mol.Pharmacol. 86 390 PMID: 25057049
Keywords: Pregnenolone sulfate sodium salt, supplier, neuroactive, steroids, highly, potent, NMDA, TRPM3, memory, LTP, potentiators, activates, activators, GABA, NMDA, Receptors, TRPM, NMDA, Receptors, Tocris Bioscience
