Biological Activity
Activator of protein kinase C; attached to cell permeabilization Antennapedia domain vector peptide. Consists of peptide derived from the C2 domain of PKC β linked by a disulfide bridge to the Antennapedia domain vector peptide. The Antennapedia peptide is actively taken up by intact cells, at 4 or 37°C, ensuring rapid and effective uptake of the activator peptide. Once inside the cell, the disulfide bonds are subjected to reduction in the cytoplasm leading to release of the activator peptide.
Technical Data
| M. Wt | 3198.81 |
| Formula | C144H225N43O34S3 |
| Sequence |
KKWKMRRNQFWIKIQRC CSVEIWD* (Modifcations: Disulfide bridge between 17 -1*) |
| Storage | Store at -20°C |
| PubChem ID | 90488710 |
| InChI Key | LTAVZZKCZYNJEE-PMCSTDROSA-N |
| Smiles | [H]N[C@@H](CSSC[C@H](N[H])C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC(O)=O)C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 2 mg/ml in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 3198.81. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.31 mL | 1.56 mL | 3.13 mL |
| 5 mM | 0.06 mL | 0.31 mL | 0.63 mL |
| 10 mM | 0.03 mL | 0.16 mL | 0.31 mL |
| 50 mM | 0.01 mL | 0.03 mL | 0.06 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Derossi et al (1994) The third helix of the antennapedia homeodomain translocates through biological membranes. J.Biol.Chem. 269 10444 PMID: 8144628
Ron and Mochly-Rosen (1995) An autoregulatory region in protein kinase C: the pseudoanchoring site. Proc.Natl.Acad.Sci.U.S.A. 92 492 PMID: 7831317
Theodore et al (1995) Intraneuronal delivery of protein kinase C pseudosubstrate leads to growth cone collapse. J.Neurosci. 15 7158 PMID: 7472470
Keywords: Pseudo RACK1, supplier, Protein, kinases, C, activators, PKC, Protein, Kinase, C, Protein, Kinase, C, Tocris Bioscience
