Biological Activity
O-GlcNAc-β-N-acetylglucosaminidase (O-GlcNAcase) and β-hexosaminidase inhibitor (Ki values are 46 and 36 nM respectively) that increases O-GlcNAc levels ~ 2-fold in HT29 cells. Z-linked isomer is more potent than the E isomer.
Technical Data
| M. Wt | 353.33 |
| Formula | C15H19N3O7 |
| Storage | Desiccate at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 132489-69-1 |
| PubChem ID | 6074876 |
| InChI Key | PBLNJFVQMUMOJY-JXAWBTAJSA-N |
| Smiles | OC(C1NC(C)=O)C(O)C(CO)O/C1=NOC(NC2=CC=CC=C2)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 35.33 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 353.33. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.83 mL | 14.15 mL | 28.3 mL |
| 5 mM | 0.57 mL | 2.83 mL | 5.66 mL |
| 10 mM | 0.28 mL | 1.42 mL | 2.83 mL |
| 50 mM | 0.06 mL | 0.28 mL | 0.57 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Dong and Hart (1994) Purification and characterization of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol. J.Biol.Chem. 269 19321 PMID: 8034696
Macauley et al (2005) O-GlcNAcase uses substrate-assisted catalysis. J.Biol.Chem. 280 25313 PMID: 15795231
Perreira et al (2006) Inhibition of O-GlcNAcase by PUGNAc is dependent upon the oxime stereochemistry. Bioorg.Med.Chem. 14 837 PMID: 16214344
Keywords: Pugnac, supplier, O-GlcNAcase, β-hexosaminidase, beta-hexosaminidase, O-GlcNAc-β-N-acetylglucosaminidase, O-GlcNAc-beta-N-acetylglucosaminidase, inhibitors, inhibits, Hydrolases, Other, Hydrolases, Other, Hydrolases, Tocris Bioscience
