Biological Activity
Potent and selective phosphodiesterase (PDE) 4 inhibitor (IC50 values are 0.28, > 100, 160 and 330 nM at PDE4, PDE1, PDE2 and PDE3 respectively). Inhibits eosinophil chemotaxis and increases progressive motility of spermatozoa in vitro.
Technical Data
| M. Wt | 411.8 |
| Formula | C19H13N5O4.HCl |
| Storage | Desiccate at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 152815-28-6 |
| PubChem ID | 19781728 |
| InChI Key | ROSFKXDQMBPYQQ-UHFFFAOYSA-N |
| Smiles | O=C(N(CC4=CC=NC=C4)C2=O)N(C3=CC([N+]([O-])=O)=CC=C3)C1=C2C=CC=N1.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 41.18 | 100 | |
| water | 20.59 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 411.8. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.43 mL | 12.14 mL | 24.28 mL |
| 5 mM | 0.49 mL | 2.43 mL | 4.86 mL |
| 10 mM | 0.24 mL | 1.21 mL | 2.43 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.49 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Alvarez et al (1995) Activation and selective inhibition of a cyclic AMP-specific phosphodiesterase, PDE-4D3. Mol.Pharmacol. 48 616 PMID: 7476886
Kaneko et al (1995) Elevated intracellular cyclic AMP inhibits chemotaxis in human eosinophils. Cell.Signal. 7 527 PMID: 8562314
Bajpai et al (2006) AKAP3 selectively binds PDE4A isoforms in bovine spermatozoa. Biol.Reprod. 74 109 PMID: 16177223
Keywords: RS 25344 hydrochloride, supplier, RS25344, potent, PDE4, inhibitors, inhibits, phosphodiesterases, Phosphodiesterases, Phosphodiesterases, Tocris Bioscience
