Biological Activity
Selective cell-permeable protein kinase C inhibitor. Displays slight selectivity for conventional PKC isoforms over Ca2+ and atypical PKC isoforms; binding affinities for rat isoforms are 9, 28, 31, 37 and 108 nM for PKC's α, βΙ, βΙΙ, γ and ε respectively. Orally available and prevents T cell chronic inflammation in vivo.
Technical Data
| M. Wt | 489.01 |
| Formula | C28H28N4O2.HCl |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 151342-35-7 |
| PubChem ID | 70346044 |
| InChI Key | HSPRASOZRZDELU-LMOVPXPDSA-N |
| Smiles | O=C(N1)C(C2=C6N(CC[C@H](CN(C)C)C6)C3=CC=CC=C23)=C(C(C5=C4C=CC=C5)=CN4C)C1=O.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.89 | 10mM with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 489.01. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.04 mL | 10.22 mL | 20.45 mL |
| 5 mM | 0.41 mL | 2.04 mL | 4.09 mL |
| 10 mM | 0.2 mL | 1.02 mL | 2.04 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.41 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Wilkinson et al (1993) Isoenzyme specificity of bisindolylmaleimides, selective inhibitors of protein kinase C. Biochem.J. 294 335 PMID: 8373348
Birchall et al (1994) Ro 32-0432, a selective and orally active inhibitor of protein kinase C prevents T-cell activation. J.Pharmacol.Exp.Ther. 268 922 PMID: 8114006
Keywords: Ro 32-0432 hydrochloride, supplier, Potent, orally, active, PKC, inhibitors, inhibits, Protein, Kinases, C, Ro32-0432, hydrochloride, Protein, Kinase, C, Protein, Kinase, C, Tocris Bioscience
