Biological Activity
ATP-competitive, potent cyclin-dependent kinase (cdk) 1 inhibitor (Ki values are 35 and 110 nM for cdk1/cyclin B1 and cdk1/cyclin A respectively). Induces G2/M phase cell cycle arrest and apoptosis. Downregulates the expression of antiapoptotic proteins such as Bcl-2 and survivin and enhances downstream p53 signaling in acute myeloid leukemia (AML).
Compound Libraries
Ro 3306 is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 351.45 |
| Formula | C18H13N3OS2 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 872573-93-8 |
| PubChem ID | 11631681 |
| InChI Key | XOLMRFUGOINFDQ-YBEGLDIGSA-N |
| Smiles | O=C2/C(SC(NCC3=CC=CS3)=N2)=C/C1=CC=C(N=CC=C4)C4=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 17.57 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 351.45. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.85 mL | 14.23 mL | 28.45 mL |
| 5 mM | 0.57 mL | 2.85 mL | 5.69 mL |
| 10 mM | 0.28 mL | 1.42 mL | 2.85 mL |
| 50 mM | 0.06 mL | 0.28 mL | 0.57 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Kojima et al (2009) The CDK1 inhibitor RO-3306 enhances p53-mediated Bax activation and mitochondrial apoptosis in AML. Cancer Sci. 100 1128 PMID: 19385969
Vassilev et al (2006) Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc.Natl.Acad.Sci. 103 10660 PMID:
Yu et al (2012) CDK1 regulates mediator of DNA damage checkpoint 1 during mitotic DNA damage. Cancer Res. 72 5448 PMID: 22962268
Keywords: Ro 3306, supplier, Ro3306, inhibits, cdk1, cyclin-dependent, kinase, 1, inhibitors, cell, cycle, arrest, apoptotic, Cyclin-dependent, Kinase, Cyclin-dependent, Kinase, Tocris Bioscience
