Biological Activity
Potent VGlut and vesicular monoamine transporter (VMAT) inhibitor (Ki values are 19 and 64 nM, respectively). Inhibits ATP dependent vesicular glutamate uptake (IC50 = 37 nM) and diminishes the amount of exocytotically released glutamate in synaptosomes. Decreases synaptic transmission in the dentate gyrus. Also stains exposed epithelium in the eye. Cell permeable.
Technical Data
| M. Wt | 1017.64 |
| Formula | C20H2Cl4I4Na2O5 |
| Storage | Store at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 632-69-9 |
| PubChem ID | 32343 |
| InChI Key | UWBXIFCTIZXXLS-UHFFFAOYSA-L |
| Smiles | O=C2C(I)=CC(C(OC3=C(I)C(O[Na])=C(I)C=C34)=C2I)=C4C1=C(C(O[Na])=O)C(Cl)=C(Cl)C(Cl)=C1Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 50.88 | 50 | |
| water | 101.76 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 1017.64. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.98 mL | 4.91 mL | 9.83 mL |
| 5 mM | 0.2 mL | 0.98 mL | 1.97 mL |
| 10 mM | 0.1 mL | 0.49 mL | 0.98 mL |
| 50 mM | 0.02 mL | 0.1 mL | 0.2 mL |
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References
References are publications that support the products' biological activity.
Pietrancosta et al (2010) Rose Bengal analogs and vesicular glutamate transporters (VGLUTs). Bioorg.Med.Chem. 18 6922 PMID: 20708942
Ogita et al (2001) Inhibition of vesicular glutamate storage and exocytotic release by Rose Bengal. J Neurochem. 77 34 PMID: 11279259
Neale et al (2013) Modulation of hippocampal synaptic transmission by the kynurenine pathway member xanthurenic acid and other VGLUT inhibitors. Neuropsychopharmacology. Neuropsychopharmacology 38 1060 PMID: 23303071
Feenstra et al (1992) What is actually stained by rose bengal? Arch.Ophthalmol. 110 984 PMID: 1637285
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