Biological Activity
Ruthenium-bipyridine-triphenylphosphine caged GABA (Cat. No. 0344) that is excited by visible wavelengths. Provides greater tissue penetration, less phototoxicity, faster photorelease kinetics and better spatial resolution than UV light-sensitive caged compounds. Produces GABA receptor-mediated currents in pyramidal neurons in vitro and displays no effect on endogenous GABAergic or glutamatergic transmission at concentrations effective for uncaging.
Licensing Information
Sold under licence from Columbia University
Technical Data
| M. Wt | 922.8 |
| Formula | C42H39F6N5O2P2Ru |
| Storage | Store at -20°C |
| CAS Number | 1028141-88-9 |
| PubChem ID | 117071637 |
| InChI Key | YMUDUHPMAODLNP-UHFFFAOYSA-N |
| Smiles | [Ru++].NCCCC([O-])=O.F[P-](F)(F)(F)(F)F.C1=CN=C(C=C1)C1=CC=CC=N1.C1=CC=C(N=C1)C1=CC=CC=N1.C1=CC=C(C=C1)P(C1=CC=CC=C1)C1=CC=CC=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility
Please refer to these solubility guidelines for RuBi-GABA (Cat.No. 3400).
Preparing Stock Solutions
The following data is based on the product molecular weight 922.8. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.08 mL | 5.42 mL | 10.84 mL |
| 5 mM | 0.22 mL | 1.08 mL | 2.17 mL |
| 10 mM | 0.11 mL | 0.54 mL | 1.08 mL |
| 50 mM | 0.02 mL | 0.11 mL | 0.22 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Zayat et al (2003) A new strategy for neurochemical photodelivery: Metal-ligand heterolytic cleavage. J.Am.Chem.Soc. 125 882 PMID: 12537482
Rial Verde et al (2008) Photorelease of GABA with visible light using an inorganic caging group. Front.Neural Circuits 2 2 PMID: 18946542
Keywords: RuBi-GABA, supplier, Caged, GABA, GABAA, GABAC, Receptors, GABAB, Caged, GABA, Compounds, GABAB, Receptors, Miscellaneous, GABA, GABAB, Receptors, Tocris Bioscience
