Biological Activity
A highly selective, potent and cell-permeable inhibitor of p38 MAP kinase. Binds within the ATP pocket of the active kinase (Kd = 38 nM, as measured in recombinant human p38), and selectively inhibits the p38α and β isoforms (IC50 = 50 and 100 nM at SAPK2a/p38 and SAPK2b/p38β2 respectively). Promotes stability of naive human pluripotent stem cells in culture. Also available as part of the MAPK Inhibitor Tocriset™.
Licensing Information
Sold with the permission of GlaxoSmithKline
Compound Libraries
SB 202190 is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 331.35 |
| Formula | C20H14N3OF |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 152121-30-7 |
| PubChem ID | 16759148 |
| InChI Key | YKRNPHOBDOUQTG-UHFFFAOYSA-N |
| Smiles | OC1=CC=C(C=C1)C1=NC(=C(N1)C1=CC=NC=C1)C1=CC=C(F)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 100 mM in DMSO |
Preparing Stock Solutions
The following data is based on the product molecular weight 331.35. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.02 mL | 15.09 mL | 30.18 mL |
| 5 mM | 0.6 mL | 3.02 mL | 6.04 mL |
| 10 mM | 0.3 mL | 1.51 mL | 3.02 mL |
| 50 mM | 0.06 mL | 0.3 mL | 0.6 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Davies et al (2000) Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem.J. 351 95 PMID: 10998351
Frantz et al (1998) The activation of p38 mitogen-activated protein kinase determines the efficiency of ATP competition for pyridinylimidazole inhibitor binding. Biochemistry 37 13846 PMID: 9753474
Jiang et al (1996) Characterization of the structure and function of a new mitogen-activated protein kinase (p38β). J.Biol.Chem. 271 17920 PMID: 8663524
Nemoto et al (1998) Induction of apoptosis by SB 202190 through inhibition of p38β mitogen-activated protein kinase. J.Biol.Chem. 273 16415 PMID: 9632706
Gafni et al (2013) Derivation of novel human ground state naive pluripotent stem cells. Nature 504 282 PMID: 24172903
Keywords: SB 202190, supplier, Potent, selective, inhibitors, inhibits, p38, MAPK, Signaling, Signalling, Mitogen-Activated, Protein, Kinases, SB202190, GlaxoSmithKline, GSK, organoids, p38, Stem, Cell, Proliferation, p38, Tocris Bioscience
