Biological Activity
Agonist peptide derived from the N-terminus of protease-activated receptor-2 (PAR2). Activates PAR2 (EC50 ~ 5 μM) and facilitates gastrointestinal transit in mice in vivo. Control peptide LRGILS-NH2 (Cat. No. 3394) also available.
Technical Data
| M. Wt | 656.82 |
| Formula | C29H56N10O7 |
| Sequence |
SLIGRL (Modifications: Leu-6 = C-terminal amide) |
| Storage | Desiccate at -20°C |
| CAS Number | 171436-38-7 |
| PubChem ID | 9831050 |
| InChI Key | SGPMJRPYYIJZPC-JYAZKYGWSA-N |
| Smiles | [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(N)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 1 mg/ml in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 656.82. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.52 mL | 7.61 mL | 15.22 mL |
| 5 mM | 0.3 mL | 1.52 mL | 3.04 mL |
| 10 mM | 0.15 mL | 0.76 mL | 1.52 mL |
| 50 mM | 0.03 mL | 0.15 mL | 0.3 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Al-Ani et al (1995) Detection of functional receptors for the proteinase-activated-receptor-2-activating polypeptide, SLIGRL-NH2, in rat vascular and gastric smooth muscle. Can.J.Physiol.Pharmacol. 73 1203 PMID: 8564891
Kawabata et al (2001) In vivo evidence that protease-activated receptors 1 and 2 modulate gastrointestinal transit in the mouse. Br.J.Pharmacol. 133 1213 PMID: 11498505
Nystedt et al (1994) Molecular cloning of a potential proteinase activated receptor. Proc.Natl.Acad.Sci.U.S.A. 91 9208 PMID: 7937743
Keywords: SLIGRL-NH2, supplier, PAR2-activating, peptide, Receptors, Protease-Activated, proteinase-activated, Protease-Activated, Receptors, Protease-Activated, Receptors, Tocris Bioscience
