Biological Activity
Proliferating cell nuclear antigen (PCNA) inhibitor; inhibits PCNA/PIP-box peptide interaction (IC50 ~1 μM). Causes DNA replication stress by stalling DNA replication forks. Inhibits PCNA interaction with DNA polymerase δ and arrests cell growth in S-phase. Suppresses cancer cell growth.
Licensing Information
Sold under license from St. Jude Childrens Research Hospital
Technical Data
| M. Wt | 511.09 |
| Formula | C15H15I2NO3 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1380782-27-3 |
| PubChem ID | 71185540 |
| InChI Key | NKOSKXJRVWVXRI-JTQLQIEISA-N |
| Smiles | IC1=C(OC2=CC=C(O)C=C2)C(I)=CC(C[C@H](N)CO)=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| 1eq. HCl | 5.11 | 10mM with gentle warming | |
| DMSO | 51.11 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 511.09. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.96 mL | 9.78 mL | 19.57 mL |
| 5 mM | 0.39 mL | 1.96 mL | 3.91 mL |
| 10 mM | 0.2 mL | 0.98 mL | 1.96 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.39 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Punchihewa et al (2012) Identification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication. J.Biol.Chem. 287 14289 PMID: 22383522
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