Biological Activity
Antiprion agent. Potently inhibits protease-resistant prion protein (PrP-res) accumulation in two types of prion-infected mouse neuroblastoma (N2a) cell lines (IC50 = 3 nM). Also reduces PrP expression and decreases proliferation and survival in schwannoma cell lines.
Technical Data
| M. Wt | 281.27 |
| Formula | C15H11N3O3 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 34320-83-7 |
| PubChem ID | 2750328 |
| InChI Key | FDAVFKZPGQEGDX-UHFFFAOYSA-N |
| Smiles | O=C(C2)N(C3=CC=CC=C3)N=C2C1=CC=C([N+]([O-])=O)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 28.13 | 100 | |
| ethanol | 7.03 | 25 |
Preparing Stock Solutions
The following data is based on the product molecular weight 281.27. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.56 mL | 17.78 mL | 35.55 mL |
| 5 mM | 0.71 mL | 3.56 mL | 7.11 mL |
| 10 mM | 0.36 mL | 1.78 mL | 3.56 mL |
| 50 mM | 0.07 mL | 0.36 mL | 0.71 mL |
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References
References are publications that support the products' biological activity.
Kimata et al (2007) New series of antiprion compounds: pyrazolone derivatives have potent activity of inhibiting protease-resistant prion protein accumulation. J.Med.Chem. 50 5053 PMID: 17850126
Provenzano et al (2017) Cellular prion protein (PrPC) in the development of Merlin-deficient tumours. Oncogene. PMID: 28692055
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