Biological Activity
Peptide fragment (residues 42-47) of protease-activated receptor 1 (PAR1) that acts as a PAR1 agonist. Stimulates platelet aggregation (EC50 = 0.8 μM), promotes intracellular Ca2+ mobilization and induces rapid phosphodiesterase 3A (PDE3A) phosphorylation in vitro.
Technical Data
| M. Wt | 748.88 |
| Formula | C34H56N10O9 |
| Sequence | SFLLRN |
| Storage | Desiccate at -20°C |
| CAS Number | 141136-83-6 |
| PubChem ID | 9831933 |
| InChI Key | HAGOWCONESKMDW-FRSCJGFNSA-N |
| Smiles | [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 1 mg/ml in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 748.88. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.34 mL | 6.68 mL | 13.35 mL |
| 5 mM | 0.27 mL | 1.34 mL | 2.67 mL |
| 10 mM | 0.13 mL | 0.67 mL | 1.34 mL |
| 50 mM | 0.03 mL | 0.13 mL | 0.27 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Vassallo et al (1992) Structure-function relationships in the activation of platelet thrombin receptors by receptor derived peptides. J.Biol.Chem. 267 6081 PMID: 1313429
Kaufmann et al (1999) PAR 1-type thrombin receptors are involved in thrombin-induced calcium signaling in human meningioma cells. J.Neurooncol. 42 131 PMID: 10421070
Hunter et al (2009) Protein kinase C-mediated phosphorylation and activation of PDE3A regulates cAMP levels in human platelets. J.Biol.Chem. 284 12339 PMID: 19261611
Keywords: TRAP-6, supplier, PAR1, peptide, fragment, residues, 42-47, agonists, Receptors, Protease-Activated, proteinase-activated, Thrombin, receptor, activator, peptide6, Thrombin, receptor, activator, peptide, 6, Protease-Activated, Receptors, Protease-Activated, Receptors, Tocris Bioscience
