Biological Activity
Selective inhibitor of protein phosphatase (PP)1 (IC50 values are 1.6 and 62 nM for PP1 and PP2 respectively). Reduces PDGF-induced vascular smooth muscle cell and mesangial cell proliferation without affecting fibronectin secretion and cellular kinase activation in vivo. Immunosuppressive agent; inhibits proliferation and induces apoptosis in activated T cells.
Technical Data
| M. Wt | 606.75 |
| Formula | C33H50O10 |
| Storage | Desiccate at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 119757-73-2 |
| PubChem ID | 16759609 |
| InChI Key | VAIBGAONSFVVKI-CIFLJJJCSA-N |
| Smiles | O=C(O1)C([C@H](O)CC(O[C@H](C)[C@@H](C)[C@H](O)CC([C@@H](C)[C@@H](O)CC[C@H](C)C[C@@H](C)CC(/C=C(CC)/C=C)=O)=O)=O)=C(C)C1=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble in DMSO (supplied pre-dissolved in DMSO, 10mg/ml) |
Preparing Stock Solutions
The following data is based on the product molecular weight 606.75. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.65 mL | 8.24 mL | 16.48 mL |
| 5 mM | 0.33 mL | 1.65 mL | 3.3 mL |
| 10 mM | 0.16 mL | 0.82 mL | 1.65 mL |
| 50 mM | 0.03 mL | 0.16 mL | 0.33 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Mitsuhashi et al (2001) Tautomycetin is a novel and specific inhibitor of serine/threonine protein phosphatase type 1, PP1. Biochem.Biophys.Res.Comm. 287 328 PMID: 11554729
Shim et al (2002) Immunosuppressive effects of tautomycetin in vivo and in vitro via T cell-specific apoptosis induction. Proc.Natl.Acad.Sci.USA. 99 10617 PMID: 12149481
Kim et al (2005) Effects of tautomycetin on proliferation and fibronectin secretion in vascular smooth muscle cells and glomerular mesangial cells. Transplant Proc. 37 1959 PMID: 15919517
Keywords: Tautomycetin, supplier, Selective, PP1, inhibitors, inhibits, Calcineurin, Protein, Ser/Thr, Phosphatases, immunosuppressants, transplant, PP2, TC, Protein, Ser/Thr, Phosphatases, Immunosuppressants, Protein, Ser/Thr, Phosphatases, Tocris Bioscience
