Biological Activity
Fluorescent form of AM 251 (Cat. No. 1117). AM 251 conjugated with 5-carboxytetramethylrhodamine (5-TAMRA) that fluoresces at 543 nm excitation (590 nm emission). Displays GPR55 binding activity.
View more information regarding Tocrifluor T1117.
Technical Data
| M. Wt | 974.97 |
| Formula | C56H53Cl2N7O5 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 1186195-59-4 |
| PubChem ID | 57369426 |
| InChI Key | RNAPRXIZPJDYPH-UHFFFAOYSA-N |
| Smiles | CN(C)C1=CC2=C(C=C1)C1(OC(=O)C3=C1C=CC(=C3)C(=O)NCCCC1=CC=C(C=C1)C1=CC=C(C=C1)C1=C(C)C(=NN1C1=CC=C(Cl)C=C1Cl)C(=O)NN1CCCCC1)C1=C(O2)C=C(C=C1)N(C)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 9.75 | 10 |
Preparing Stock Solutions
The following data is based on the product molecular weight 974.97. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.03 mL | 5.13 mL | 10.26 mL |
| 5 mM | 0.21 mL | 1.03 mL | 2.05 mL |
| 10 mM | 0.1 mL | 0.51 mL | 1.03 mL |
| 50 mM | 0.02 mL | 0.1 mL | 0.21 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Daly et al (2010) Fluorescent ligand binding reveals heterogeneous distribution of adrenoceptors and 'cannabinoid-like' receptors in small arteries. Br.J.Pharmacol. 159 787 PMID: 20136833
Daly et al (2008) Visualisation of vascular cannabinoid receptors and their potential interaction with α1-adrenergic receptors. Vascular and Smooth Muscle Phys.Soc. Symposium PMID:
Sylantyev et al (2013) Cannabinoid- and lysophosphatidylinositol-sensitive receptor GPR55 boosts neurotransmitter release at central synapses. Proc.Natl.Acad.Sci.U.S.A. 110 5193 PMID: 23472002
Bruno et al (2014) Beyond radio-displacement techniques for identification of CB1 ligands: the first application of a fluorescence-quenching assay. Sci.Rep. 4 3757 PMID: 24441508
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