Biological Activity
Negative control of UNC 1999 (Cat. No. 4904). Exhibits 1000-fold lower potency than active analog (IC50 values are 62 and >200 μM for EZH1 and EZH2, respectively).
Licensing Information
This compound is supplied in conjunction with the Structural Genomics Consortium. For further characterization details, please visit the UNC 1999 probe summary on the SGC website.
Technical Data
| M. Wt | 597.79 |
| Formula | C35H47N7O2 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1433200-49-7 |
| PubChem ID | 91691116 |
| InChI Key | IFSQHIRDVVFJSQ-UHFFFAOYSA-N |
| Smiles | CC(C)N1CCN(C2=NC=C(C3=CC(N(C(C)C)N=C4)=C4C(C(N(C)CC5=C(CCC)C=C(C)N(C)C5=O)=O)=C3)C=C2)CC1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 100 mM in DMSO and to 100 mM in 1eq. HCl |
Preparing Stock Solutions
The following data is based on the product molecular weight 597.79. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.67 mL | 8.36 mL | 16.73 mL |
| 5 mM | 0.33 mL | 1.67 mL | 3.35 mL |
| 10 mM | 0.17 mL | 0.84 mL | 1.67 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.33 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Konze et al (2013) An orally bioavailable chemical probe of the lysine methyltransferases EZH2 and EZH1. ACS Chem.Biol. 8 1324 PMID: 23614352
Xu et al (2015) Selective inhibition of EZH2 and EZH1 enzymatic activity by a small molecule suppresses MLL-rearranged leukemia. Blood 125 346 PMID: 25395428
Keywords: UNC 2400, supplier, UNC2400, Negative, controls, UNC1999, EZH1, EZH2, histone, methyltransferase, PRC2, polycomb, repressive, complex, 2, Lysine, Methyltransferases, Lysine, Methyltransferases, Lysine, Methyltransferases, Tocris Bioscience
