Biological Activity
Calmodulin antagonist. Inhibits Ca2+-calmodulin-dependent phosphodiesterase (IC50 = 28 μM) and myosin light chain kinase (IC50 = 51 μM).
Technical Data
| M. Wt | 377.33 |
| Formula | C16H21ClN2O2S.HCl |
| Storage | Store at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 61714-27-0 |
| PubChem ID | 124887 |
| InChI Key | OMMOSRLIFSCDBL-UHFFFAOYSA-N |
| Smiles | Cl.NCCCCCCNS(=O)(=O)C1=CC=CC2=C(Cl)C=CC=C12 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 37.73 | 100 | |
| water | 1.89 | 5mM with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 377.33. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.65 mL | 13.25 mL | 26.5 mL |
| 5 mM | 0.53 mL | 2.65 mL | 5.3 mL |
| 10 mM | 0.27 mL | 1.33 mL | 2.65 mL |
| 50 mM | 0.05 mL | 0.27 mL | 0.53 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Asano et al (1989) Divergent pharmacological effects of three calmodulin antagonists, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W 7), chlorpromazine and calmidazolium, on isometric tension development and myosin light chain phosphorylation in intact bovine trachea J.Pharmacol.Exp.Ther. 251 764 PMID: 2810127
Hidaka et al (1981) N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation. Proc.Natl.Acad.Sci.U.S.A. 78 4354 PMID: 6945588
Itoh and Hidaka (1984) Direct interaction of calmodulin antagonists with Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase. J.Biochem. 96 1721 PMID: 6099352
Tanaka and Hidaka (1980) Hydrophobic regions function in calmodulin-enzyme(s) interactions. J.Biol.Chem. 255 11078 PMID: 6254958
Keywords: W-7 hydrochloride, supplier, Calmodulin, antagonists, inhibitors, inhibits, myosin, light, chain, kinases, Ca2+, Binding, Protein, modulators, Calcium, Signaling, Signalling, MLCK, Ca2+-calmodulin-dependent, phosphodiesterase, Calcium, Binding, Protein, Myosin, Light, Chain, Kinase, Myosin, Calcium, Binding, Protein, Tocris Bioscience
