Biological Activity
Selective agonist for the formyl peptide receptors FPR1, FPR2 (EC50 = 75 pM) and FPR3 (EC50 = 3 nM), expressed on immune cells. Induces Ca2+ mobilization and superoxide production in, and chemotaxic migration of, monocytes and neutrophils. Also promotes monocyte survival through a PKC-, PI 3-kinase- and Akt-dependent pathway.
Technical Data
| M. Wt | 856.11 |
| Formula | C41H61N9O7S2 |
| Sequence |
WKYMVM (Modifications: Met-6 = D-Met & C-terminal amide) |
| Storage | Desiccate at -20°C |
| CAS Number | 187986-17-0 |
| PubChem ID | 457933 |
| InChI Key | FMBGOORJEKQQLG-JUZZZACGSA-N |
| Smiles | [H]N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](CCSC)C(N)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 2 mg/ml in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 856.11. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.17 mL | 5.84 mL | 11.68 mL |
| 5 mM | 0.23 mL | 1.17 mL | 2.34 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.17 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Bae et al (2002) The synthetic chemoattractant peptide, Trp-Lys-Tyr-Met-Val-D-Met, enhances monocyte survival via PKC-dependent Akt activation. J.Leukoc.Biol. 71 329 PMID: 11818455
Christophe et al (2001) The synthetic peptide Trp-Lys-Tyr-Met-Val-Met-NH2 specifically activates neutrophils through FPRL1/Lipoxin A4 receptors and is an agonist for the orphan monocyte-expressed chemoattractant receptor FPRL2. J.Biol.Chem. 276 21585 PMID: 11285256
Le et al (1999) Utilization of two seven-transmembrane, G protein-coupled receptors, formyl peptide receptor-like 1 and fomyl peptide receptor, by the synthetic hexapeptide WKYMVm for human phagocyte activation. J.Immunol. 163 6777 PMID: 10586077
Keywords: WKYMVm, supplier, FPR1, FPR2, FPR3, receptor, agonists, FPRL, Formyl, like, Peptide, Receptors, Leukocyte, chemoattractant, peptide, Formyl, Peptide, Receptors, Formyl, Peptide, Receptors, Tocris Bioscience
