Biological Activity
Cell-permeable reactive oxygen species scavenger that activates p53 through a JNK-dependent signaling pathway. Activates p21waf-1 and MDM2, represses transcription of Myc and thymidine kinase, and inhibits DNA topoisomerase IIα. Induces cell cycle arrest and exhibits cytoprotective activity towards normal cells but not cancer cells in vivo. Also exhibits broad spectrum antiviral activity in vitro.
Technical Data
| M. Wt | 207.16 |
| Formula | C5H14N2S.2HCl |
| Storage | Desiccate at -20°C |
| CAS Number | 14653-77-1 |
| PubChem ID | 146169 |
| InChI Key | XDRLRDHLCIFZIW-UHFFFAOYSA-N |
| Smiles | NCCCNCCS.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 20.72 | 100 | |
| water | 20.72 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 207.16. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.83 mL | 24.14 mL | 48.27 mL |
| 5 mM | 0.97 mL | 4.83 mL | 9.65 mL |
| 10 mM | 0.48 mL | 2.41 mL | 4.83 mL |
| 50 mM | 0.1 mL | 0.48 mL | 0.97 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
North et al (2000) The cytoprotective aminothiol WR1065 activates p21waf-1 and down regulates cell cycle progression through a p53-dependent pathway. Oncogene 19 1206 PMID: 10713709
Pluquet et al (2003) The cytoprotective aminothiol WR1065 activates p53 through a non-genotoxic signaling pathway involving c-Jun N-terminal kinase. J.Biol.Chem. 278 11879 PMID: 12531896
Walker et al (2009) WR1065 mitigates AZT-ddl-induced mutagenesis and inhibits viral replication. Enviorn.Mol.Mutagen. 50 460 PMID:
Keywords: WR 1065, supplier, WR, 1065, dihydrochloride, p53, activators, ROS, scavenger, Apoptosis, Cell, Cycle, Transcription, Factors, reactive, oxygen, species, WR1065, p53, Antivirals, p53, Tocris Bioscience
