Biological Activity
Selective inhibitor of Cdc42. Targets the binding site of the Cdc42 guanine nucleotide exchange factor, intersectin (ITSN). Inhibits Cdc42-mediated cellular effects, including microspike formation in 3T3 fibroblasts and neuronal branching in primary neonatal cortical neurons. Also suppresses cell motility and migration in PC3 cells, without cytotoxic effects.
Compound Libraries
ZCL 278 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 584.89 |
| Formula | C21H19BrClN5O4S2 |
| Storage | Store at +4°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 587841-73-4 |
| PubChem ID | 1791111 |
| InChI Key | XKZDWYDHEBCGCG-UHFFFAOYSA-N |
| Smiles | ClC1=C(OCC(NC(NC2=CC=C(S(NC3=NC(C)=CC(C)=N3)(=O)=O)C=C2)=S)=O)C=CC(Br)=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 58.49 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 584.89. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.71 mL | 8.55 mL | 17.1 mL |
| 5 mM | 0.34 mL | 1.71 mL | 3.42 mL |
| 10 mM | 0.17 mL | 0.85 mL | 1.71 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.34 mL |
Molarity Calculator
Reconstitution Calculator
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Product Datasheets
References
References are publications that support the products' biological activity.
Friesland et al (2013) Small molecule targeting Cdc42-intersectin interaction disrupts Golgi organization and suppresses cell motility. Proc.Natl.Acad.Sci.USA 110 1261 PMID: 23284167
Keywords: ZCL 278, supplier, ZCL278, Cdc42, Rho, small, GTPases, inhibitors, inhibits, G, Proteins, (Small), GTPase, GTPase, Tocris Bioscience
