Biological Activity
Sodium-hydrogen exchanger isoform 1 (NHE1) inhibitor that displays selectivity over other NHE isoforms (Ki values are 14, 2200 and 220000 nM for human NHE1, human NHE2 and rat NHE3 respectively). Inhibits NHE1 dependent 22Na+ uptake in vitro (IC50 = 14 nM) and provides cardioprotection from myocardial ischemic injury in vivo (EC50 = 0.25 nM). Also inhibits MMP2/9 activity and invasion in breast cancer cells.
Licensing Information
Sold for research purposes under agreement from Pfizer Inc
Compound Libraries
Zoniporide dihydrochloride is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 393.27 |
| Formula | C17H16N6O.2HCl |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 241799-10-0 |
| PubChem ID | 22267855 |
| InChI Key | ARZNPJAGKWHEHT-UHFFFAOYSA-N |
| Smiles | O=C(/N=C(N)/N)C(C=N3)=C(C4CC4)N3C1=C2C(N=CC=C2)=CC=C1.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 39.33 | 100 | |
| water | 3.93 | 10 |
Preparing Stock Solutions
The following data is based on the product molecular weight 393.27. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.54 mL | 12.71 mL | 25.43 mL |
| 5 mM | 0.51 mL | 2.54 mL | 5.09 mL |
| 10 mM | 0.25 mL | 1.27 mL | 2.54 mL |
| 50 mM | 0.05 mL | 0.25 mL | 0.51 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Knight et al (2001) A novel sodium-hydrogen exchanger isoform-1 inhibitor, zoniporide, reduces ischemic myocardial injury in vitro and in vivo. J.Pharmacol.Exp.Ther. 297 254 PMID: 11259552
Marala et al (2002) Zoniporide: a potent and highly selective inhibitor of human Na+/H+ exchanger-1. Eur.J.Pharmacol. 451 37 PMID: 12223226
Clements-Jewery et al (2004) Cardioprotective efficacy of zoniporide, a potent and selective inhibitor of Na+/H+ exchanger isoform-1, in an experimental model of cardiopulmonary bypass. Br.J.Pharmacol. 142 57 PMID: 15037516
Keywords: Zoniporide dihydrochloride, supplier, Selective, NHE-1, inhibitors, inhibits, Ion, Pumps, Transporters, Sodium-hydrogen, exchanger, isoform-1, CP597396, CP, 597396, Na+/H+, Exchanger, Na+/H+, Exchanger, Tocris Bioscience
