Biological Activity
Smoothened (Smo) receptor antagonist (IC50 = 0.5 μM in HEK293 cells transiently expressing mouse Smo). Inhibits ShhN-induced Gli luciferase reporter activity in Shh-light 2 cells (IC50 = 2.5 μM); acts as an inverse agonist during nontranscriptional Hedgehog pathway activation (IC50 = 2.5 μM in HEK293 cells).
Technical Data
| M. Wt | 465.52 |
| Formula | C24H23N3O5S |
| Storage | Store at +4°C |
| Purity | ≥98% |
| CAS Number | 330829-30-6 |
| PubChem ID | 1139102 |
| InChI Key | KVQVEZQDNHMQJV-UHFFFAOYSA-N |
| Smiles | O=C(NC(NC2=CC(NC(C3=CC=CC=C3)=O)=CC=C2)=S)C1=CC(OC)=C(OC)C(OC)=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| DMSO | 46.55 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 465.52. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 1 mM | 2.15 mL | 10.74 mL | 21.48 mL |
| 5 mM | 0.43 mL | 2.15 mL | 4.3 mL |
| 10 mM | 0.21 mL | 1.07 mL | 2.15 mL |
| 50 mM | 0.04 mL | 0.21 mL | 0.43 mL |
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Certificate of Analysis / Product Datasheet Safety Datasheet Select another language: English German French Spanish View SDSReferences
References are publications that support the products\' biological activity.
Solinas et al (2012) Acylthiourea, acylurea, and acylguanidine derivatives with potent hedgehog inhibiting activity. J.Med.Chem. 55 1559 PMID: 22268551
Manetti et al (2010) Virtual screening-based discovery and mechanistic characterization of the acylthiourea MRT-10 family as smoothened antagonists. Mol.Pharmacol. 78 658 PMID: 20664000
Keywords: MRT 10, supplier, MRT10, smoothened, receptor, antagonists, inhibitors, inhibits, hedgehog, pathway, inverse, agonists, nontranscriptional, Gli, Shh, sonic, Smoothened, Receptors, Hedgehog, Signaling, Smoothened, Receptors, Tocris Bioscience
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